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(More customer reviews)This book is really a great help for beginners of basic research in the HIV field. It not only covers the technical approaches for conducting research on HIV but also it gives a background on basic knowledge as well as safety measures during handling HIV in research laboratory.
Click Here to see more reviews about: HIV: A Practical Approach Volume 2: Biochemistry, Molecular Biology, and Drug Discovery (Practical Approach Series)
HIV Volume 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described as well as assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery. Contents: The HIV life cycle; Reverse transcriptase; Ribonulease H; Integrase; Protease; Expression of gag, envelope and other HIV antigens using Bacculovirus; Envelope expression and purification; HIV envelope glyocoprotein/CD4 interactions - studies using recombinant vaccinia virus vectors; RNA binding assays for the regulatory proteins tat and rev; Cellular and cell-free assays for tat; Cellular assays for rev; DNA binding proteins interacting with the viral LTR; Nef; Vpu; Vif; Cellular assays for antiviral drugs; Inhibition of HIV by ribozymes and antisense oligonucleotides; Gene therapy approaches to the inhibition of HIV
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